IC50 and EC50 Calculator (Dose-Response Curve)

Fit a four-parameter logistic online and get the IC50 or EC50 with 95% CI, Hill slope and asymptotes. Downloadable PNG curve, no GraphPad install needed.

How to cite this tool

If you use it in your thesis or in a paper, cite it so that readers of your Methods section know how the calculation was done.

Ciudad-Fernández, V. (2026). Dose-response curve calculator [Web application]. Ciudad Science. Retrieved October 14, 2026, from https://analisisdedatospsicologia.com/en/tools/dose-response-curve-calculator

For your Methods section: The dose-response curve was fitted with a four-parameter logistic (Hill) model, from which the EC50 or IC50 was obtained, using the Ciudad Science dose-response curve calculator (Ciudad-Fernández, 2026).

@misc{ciudadfernandez2026dosisrespuesta,
  author       = {Ciudad-Fern{\'a}ndez, V{\'i}ctor},
  title        = {{Dose-response curve calculator}},
  year         = {2026},
  publisher    = {Ciudad Science},
  howpublished = {Web application},
  url          = {https://analisisdedatospsicologia.com/en/tools/dose-response-curve-calculator},
  urldate      = {2026-10-14},
  note         = {Accessed 2026-10-14},
}

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